Stomatologist № 4 (31) – 2018, pp. 29-33                                                                                                                                 Scientific publication


Microorganisms forming biofilm in the pathogenesis of sialadenitis


V.K. Okylicha, A.I. Goncharovab

aMD, PhD, Associate Professor, Vitebsk State Medical University, Vitebsk, Belarus
bVitebsk State Medical University, Vitebsk, Belarus

https://doi.org/10.32993/stomatologist.2018.4(31).5

ABSTRACT
The aim of the study is to determine the role of microorganisms forming biofilm in the pathogenesis of sialadenitis.
Objects and methods. Totally 86 patients with sialadenitis were examined. Patients were on inpatient treatment in the Vitebsk Regional Clinical Hospital. Isolation and identification of ductal saliva microorganisms among patients with sialadenitis were carried out. The ability of isolates to form biofilms was determined. Sensitivity assessment of the isolated microorganisms to antibiotics in biofilm was performed.
Results and discussion. The significance of the microbial factor was confirmed in 77.9 per cent of cases. The representatives of the genus Streptococcus (45.8%) and the genus Staphylococcus (32.9%) prevailed among the identified isolates. The representatives of the species S. aureus – 24.45; 20.27 – 49.29 μg / socket had the greatest ability to form a microbial film among the frequently occurring isolates. An increased МIК90 from 8,5 to 99 times was determined due to the comparison of MIK90 S. aureus in plankton and biofilm forms. Imipenem, meropenem and vancomycin were the most effective against S. aureus.
Conclusions.. The etiological structure of sialadenitis was studied. The ability of the selected isolates to form biofilms was determined. The sensitivity of the isolated microorganisms to antibiotics in the composition of the biofilm was evaluated.

Keywords: sialadenitis, biofilm, antibiotics

References

  1. Obolenskiy V.N. [et al.] Antibiotikoprofilaktika, antibiotikoterapiya i mikrobiologicheskaya situatsiya v hirurgicheskom statsionare [Antibiotic prophylaxis, antibiotic therapy and the microbiological situation in the surgical hospita]. Antibiotiki i himioterapiya – Antibiotics and Chemotherapy, 2004, vol. 49, no. 10, pp. 13–19.
  2. Kabanova A.A. Pokazateli mikrotsirkulyatsii u patsientov s flegmonami chelyustno-litsevoy oblasti [The microcirculation parameters at the patient with phlegmons of the maxillofacial area]. Stomatolog. Minsk. – Stomatologist, 2016, no. 1 (20), pp. 66–68.
  3. Kabanova A.A., Goncharova A.I., Kabanova S.A. BAPNA-amidaznaya i elastaznaya aktivnost rotovoy zhidkosti patsientov s gnoyno-vospalitelnyimi zabolevaniyami chelyustno-litsevoy oblasti. [BAPNA-amidase and elastase activity of the oral fluid of patients with purulent-inflammatory diseases of the maxillofacial region]. Minsk. – Stomatologist, Minsk. 2014, no. 2 (13), pp. 7–10.
  4. Miranovich S.I., Petrovskiy E.V. Bakteriologicheskaya harakteristika flegmon chelyustno-litsevoy oblasti [Bacteriological characteristics of maxillofacial phlegmon]. Minsk. – Stomatologist, Minsk. 2013, no. 1 (8), pp. 69–72.
  5. Pohodenko-Chudakova I.O., Ignatovich A.P. Sravnitelnaya otsenka effektivnosti lekarstvennyih sredstv, ispolzuemyih dlya profilaktiki oslozhneniy pri ambulatornyih hirurgicheskih vmeshatelstvah v polosti rta [Comparative evaluation of the effectiveness of drugs used to prevent complications of outpatient surgical interventions in the oral cavity]. Minsk. – Stomatologist, Minsk. 2012, no. 2 (5), pp. 43–46.
  6. Balaban N.Q. [et al.] Bacterial Persistence as a Phenotypic Switch. Science, 2004, vol. 305, no. 5690, pp. 1622–1625.
  7. Rice S.A. [et al.] Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues. J. Bacteriol, 2005, no. 187, pp. 3477–3485.
  8. Cassat J. [et. al.] Transcriptional profiling of a Staphylococcus aureus clinical isolate and its isogenic agr and sarA mutants reveals global differences in comparison to the laboratory strain RN6390. Microbiology, 2006, no. 152, pp. 3075–3090.
  9. Davey M.E., O’Toole G.A. Microbial biofilms: from ecology to molecular genetics. Microbiol. Mol. Biol. Rev., 2000, no.64, pp. 847–867.
  10. Donlan R.M., Costerton J.W. Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms. CLIN. MIC. REV., 2002, vol. 15, no. 2, pp. 167–193.
  11. Shen Y. [et al.] Effect of ambient humidity on the strength of the adhesion force of single yeast cell inside environmental-SEM. Ultramicroscopy, 2011, vol. 111, no. 8, pp. 1176–1183.
  12. Palmer R.J.Jr., Stoodley P. Biofilms 2007: broadened horizons and new emphases. J. Bacteriol., 2007, no. 189 (22), pp. 7948–7960.
  13. Stewart P.S., Costerton J.W. Antibiotic resistance of bacteria in biofilms Lancet, 2001, no. 358, pp. 135–138.

Correspondence to:  Е-mail: vokul@mail.ru