Stomatologist № 2 (33) – 2019, pp. 50-55                                                                                                                                 Scientific publication

Application of cell biotechnologies in the treatment of gum recession

S.P. Rubnikovicha, I.D. Volotovskyb, Yu.L. Denisovac, L.N. Dedovac, V.A. Andreevad, G.Yu. Panasenkovae, T.P. Novikf

aMD, PhD, DMSci, Professor, Belarusian Medical Academy of Postgraduate Education, Minsk, Belarus
bPhD, DBiolSci, Professor, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, Belarus
cMD, PhD, DMSci, Professor, Belarusian State Medical University, Minsk, Belarus
dMD, PhD, Associate Professor, Belarusian Medical Academy of Postgraduate Education, Minsk, Belarus
eBelarusian Medical Academy of Postgraduate Education, Minsk, Belarus
fBelarusian State Medical University, Minsk, Belarus

https://doi.org/10.32993/stomatologist.2019.2(33).7

ABSTRACT
The aim of the study is to develop and scientifically justify a new method for treating patients with gingival recession using autologous mesenchymal stem cells of adipose tissue.
Objects and methods. A complex treatment was conducted in 93 patients with an anatomical recession of the gums in combination with dentoalveolar anomalies at the age of 20 to 34 years. Patients were divided into 3 groups (1st, 2nd and 3rd groups) depending on the treatment used. All patients underwent periodontological treatment. In the second group, patients included periodontological treatment including orthodontic equipment, in the third group, orthodontic treatment and the use of a cell transplant on the basis of a mixture of autologous mesenchymal stem cells and sterile bioplastic collagen material “Collost”.
Results and discussion. A comprehensive assessment of the periodontal tissue of the three groups showed that among the patients who received the standard treatment complex, a good condition of the periodontal tissues 1 to 2 years after treatment was noted only in 16.7% of the patients of the 1st group. Inclusion of orthodontic treatment made it possible to obtain good results in 25% of patients in the 2nd group, which is 8% more than in the 1st group. At the same time, good results of treatment were revealed in 94% of patients in group 3, who included periodontological and orthodontic treatment using autologous mesenchymal stem cells, which confirms the need for measures aimed at normalization position of teeth, dentition and bite with the use of small orthodontic long-acting forces, improving microcirculation of periodontal tissues and restoring the contour of the marginal gum.
Conclusions. Inclusion of autologous mesenchymal stem cells in the complex treatment of patients with gingival recession is accompanied by a 1.6-fold decrease in the prevalence and gum recession (1.15 mm), and tissue volume increased 2.6-fold, stimulation of periodontal microcirculation processes (in terms of capillary the periodontal pressure was 1.1 times and the intensity of periodontal microcirculation was 3.1 times), as well as the normalization of the position of teeth, dentition and occlusion, which allowed to obtain good long-term results in 94% of patients.

Keywords: mesenchymal stem cells, gum recession, cellular technology

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Correspondence to:  Е-mail: rubnikovichs@mail.ru