Dentist № 4 (39) – 2020, pp. 16-23                                                                                                   SCIENTIFIC PUBLICATION

Activation of neutrophils and lymphocytes by components dental material

I.Yu. Karpuk

MD, PhD, DMSci, Professor, Vitebsk State Medical University, Vitebsk, Belarus

https://doi.org/10.32993/dentist.2020.4(39).5

ABSTRACT
The aim of the study was to evaluate the release of myeloperoxidase and elastase from the neutrophils and lactoperoxidase from the salivary glands into the oral fluid after oral-buccal provocative tests with components of dental materials to diagnose hypersensitivity to them.
Objects and methods. The study group consisted of 22 patients with intolerance to dental materials. Patients underwent an oral-buccal provocative test with 0.001% solution of metal salts: NiCl2, CrCl3, CoCl2.
Results and discussion. It was found that an increase in the level of peroxidase and elastase activity in the oral fluid was observed in patients with intolerance to dental materials after an oral-buccal provocation test with a solution of metal salts, but not in patients of the control group. Activation of CD4 + T-lymphocytes in response to stimulation by PFF and MSS was detected. As an outcome of the study, a number of regularities in the expression of markers for T-lymphocytes and NK-lymphocytes activation were established. The results confirm the potential role of CD4+ CD25+CD45+ T-lymphocytes and CD16+CD56+CD69+ NKlymphocytes in the development of hypersensitivity to CDM.
Conclusion. The measurement of allergen-induced total peroxidase activity in the oral fluid can be a promising new diagnostic method for confirming the allergenicity of dental constructions. The method of CDM hypersensitivity diagnosis by measuring increasing CD4+CD25+CD45+ T-lymphocytes and CD16+CD56+CD69+NK-lymphocytes using PFF and MSS as stimulants can serve as a reliable test for the diagnosis of intolerance to dental materials.

Keywords: oral fluid, intolerance, dental materials, myeloperoxidase, lactooperoxidase, T-lymphocytes, NK lymphocytes

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Correspondence to:  Е-mail: ikarpuk@mail.ru