Dentist № 1 (48) – 2023, pp. 20-25                                                                                                             SCIENTIFIC PUBLICATION

Modern concept of experimental research on biological models in dental implantology

I.O. Pohodenko-Chudakovaa, T.L. Shevelab

aMD, PhD, DMSci, Professor, Belarusian State Medical University, Minsk, Belarus
bMD, PhD, Associate Professor, Belarusian State Medical University, Minsk, Belarus

https://doi.org/10.32993/dentist.2023.1(48).7

ABSTRACT
The most important component of scientific research is an experiment based on experience with precisely accounted and controlled conditions. The significance of the experiment performed with the involvement of animals is great, since animals represent the most optimal model of the human body.
The aim of the study is to derive a modern concept of experimental research conducted on biological models in dental implantology based on the analysis of own experimental data and information from the special literature presented in the periodical press in the last 25 years.
Objects and methods. The experiment was made on 10 individuals of male Chinchilla rabbits. The types of bone tissue of the experimental animals were determined on basis of the Hounsfield index determined by the results of cone-beam computed tomography. The special literature containing information on experimental studies on dental implantation was analyzed: periodicals for the last 25 years, basic manuals and monographs without a statute of limitations.
Results and discussion. Based on the Housefield index determined by the results of cone-beam computed tomography, 4 types of bone tissue were determined in the skeleton of experimental rabbit objects.
Conclusion. The results of the analysis of the information in the special literature and own data obtained under experimental conditions allowed us to find out the modern concept of experimental research in dental implantology which allows taking into account the type of bone tissue, using a Chinchilla rabbit as a biological object for preclinical studies.

Keywords: experimental model, biological object, process research, dental implantation, laboratory animals, osseointegration

References 

  1. Grigorʼian A.S., Khamraev T.K., Toporkova A.K., Amirov A.R. Jeksperimental’noe issledovanie integracii v kostnuju tkan’ dental’nyh implantatov s nanostrukturirovannym nerezorbiruemym pokrytiem [Experimental study of dental implanta with nanostructured non-resorbable coating integration into bone tissue]. Stomatologiya. – Dentistry. 2010, vol. 89, no. 4, pp. 14-17.
  2. Gudaryan A.A., Shirinkin S.V. Sovremennye podhody v kompleksnom lechenii vospalitel’no-destruktivnyh oslozhnenij dental’noj implantacii [Modern approaches in treatment of periimplant mucositis and periimplantitis]. Sciences of Europe, 2019, vol. 36, no. 2, pp. 55-63.
  3. Denisov A.V., Cheprakova V.A., Anisin A.V. Bezrukov S.I. Jeticheskie aspekty ispol’zovanija zhivotnyh v sovremennyh jeksperimental’nyh issledovanijah [Ethical aspects of modern use of animals in experimental studies]. Vestnik Ros. Voenno-med. Akademii. ‒ Bull. of the Rus. Military Medical Academy. 2018, vol. 63, no. 3, pp. 238-242, doi: 17816/brmma12383
  4. Karkishhenko N.N. Al’ternativy biomediciny. Tom 1. Osnovy biomediciny i farmakomodelirovanija [Alternatives to biomedicine. Vol. 1. Fundamentals of biomedicine and pharmacomodeling]. Moscow: Publishing House of the Military Industrial Complex, 2007. 320 p.
  5. Korel’ A.V., Kuznecov S.B. Tkaneinzhenernye strategii dlja vosstanovlenija defektov kostnoj tkani. Sovremennoe sostojanie voprosa [Tissue engineering strategies for the restoration of bone defects. Current state of the issue]. Mezhdunarod. zhurn. prikladnyh i fundamental’nyh issled. – Internat. J. of Appl. and Fundamental Res. 2019, no. 4, pp. 228-234.
  6. Kosach G.A., Kutukova S.I., Vlasov T.D., Yaremenko A.I. Biomarkery kostnoj regeneracii v cheljustno-licevoj hirurgii: kriticheskij obzor literatury [Bone regeneration biomarkers in maxillofacial surgery: a critical review of literature]. Parodontologiya. – Periodontology. 2021, vol. 26, no. 4, pp. 284-288, doi: 10.33925/1683-3759-2021-26-4-284-288
  7. Pohodenko-Chudakova I.O., Surin A.V. Obosnovanie vybora krolika jeksperimental’nym ob#ektom dlja modelirovanija hronicheskogo sinusita verhnecheljustnoj pazuhi [Basis for the selection of rabbit for experimental object for simulation chronic sinusitis of the maxillary sinus]. Stomatologija. Jestetika. Innovacii. – Dentistry. Aesthetics. Innovations. 2019, vol. 3, no. 1, pp. 38-47.
  8. Pohodenko-Chudakova I.O., Groshev E.Yu. Obosnovanie ispol’zovanija krovlika porody Shinshilla dlja sozdanija jeksperimental’noj modeli travmaticheskogo povrezhdenija licevogo nerva [Justification for the use of a Shinshilla rabbit to create an experimental model of traumatic facial nerve injury]. Sovremennaja stomatologija. – Modern dentistry. 2022, vol. 88, no. 3, pp. 39-43.
  9. Pohodenko-Chudakova I.O., Мaksimovich E.V. Jeksperimental’noe issledovanie gepatotoksichnosti 2 % rastvora lidokaina gidrohlorida pri razlichnom chisle in#ekcij [The experimental trials of 2 % lidocaine hydrochloride solution hepatotoxicity for different number of injections]. Visnik problem biologii i medicini – Bull. of Problems of Biology and Medicine. 2018, no. 3 (145), vol. 1, pp. 318-321, doi: 10.29254/2077-4214-2018-3-145-318-321
  10. Skripnikova I.A., Shhepljagina L.A., Novikov V.E., Kosmatova O.V., Abirova A.S. Vozmozhnosti kostnoj rentgenovskoj densitometrii v klinicheskoj praktike [Possibilities of bone X-ray densitometry in clinical practice]. Osteoporoz i osteopatii. – Osteoporosis and Bone Diseases. 2010, no. 2, pp. 23-25.
  11. Ushakov A.I., Yurʼev E.M. Dental’naja implantacija i vybor kostno-plasticheskih materialov v zavisimosti ot tipa kostnoj tkani cheljustej [Dental implantation and the choice of the osteoplastic materials taking into consideration the type of the bone tissue of human jaws]. Rossijskaja stomatologija. – Russian Stomalology. 2016, vol. 9, no. 2, pp. 12-17, doi: 10.17116/rosstomat20169212-17
  12. Hajkin M.B., Simahov R.V., Chigarina S.E., Grishin O.P., Kalinnikova E.A., Supil’nikov A.A. Analiz jeksperimental’nyh i klinicheskih issledovanij ispol’zovanija metoda neposredstvennoj dental’noj implantacii [Analysis of experimental and clinical studies of the use of the method of direct dental implantation]. Vestnik med. instituta «REAVIZ». – Bull. Med. Institute “Rehabilitation, Doctor and Health”. 2019, vol. 38, no. 2, pp. 141-148.
  13. Hafizov R.G., Azizova D.A., Zhitko A.K., Zhitko R.K. Dental’naja implantologija. Osnovy: ucheb.-metod. posobie [Dental implantology. Basics: Edu. and Method. Manual]. Kazan: Kazan Federal University, 2021. 57 p.
  14. Khismatullina Z.N. Faktory, okazyvajushhie vlijanie na metabolizm kostnoj tkani i privodjashhie k zabolevanijam kostnoj sistemy [Factors affecting the metabolism of bone tissue and leading to diseases of the bone system]. Vestnik tehnologicheskogo universiteta. – Bull. of the Technological University, 2015, vol. 18, no. 22, pp. 165-172.
  15. Homutov A.E. Krylova E.V., Kopylova S.V. Anatomija cheloveka. Chast’ 1. Osteologija: ucheb. posobie [Human anatomy. Part 1. Osteology: Textbook]. N. Novgorod: Publishing House of UNN, 2015. 153 p.
  16. Chadayev V.Yu. Jeticheskie principy pri rabote s laboratornymi zhivotnymi [Ethical principles when working with laboratory amimals]. Visnik problem biologii i medicini. – Bull. of Probl.of Biology and Med. 2012, vol. 92, no. 2, pp. 113-115.
  17. Oettinger A.P., Zharova M.E. Chto takoe dokazatel’naja medicina? [What is evidence-based medicine]. Dokazatel’naja gastrojenterologija. – Rus. J. of Evidence-based Gastroenterology, 2021, vol.10, no. 1, pp. 38-48, doi: 10.17116/dokgastro20211001138
  18. Ahmad O.K., Kelly J.R. Assessment of the primary stability of dental implants in artificial bone using resonance frequency and percussion analyses. Int. J. Oral Maxillofac. Implants, 2013, vol. 28, no. 1, pp. 89-95, doi: 11607/jomi.2554
  19. Di StefanoA. [et al.] Stability of dental implants and thickness of cortical bone: clinical research and future perspectives. A systematic review. Materials (Basel), 2021, vol. 23, no. 14, 7183 p., doi: 10.3390/ma14237183
  20. Fischer B. [et al.] Rabbit as a reproductive model for human health. Reproduction, 2012, vol. 144, no. 1, pp. 1-10, doi: 10.1530/REP-12-0091
  21. Hosseini-Faradonbeh S.A., Katoozian H.R. Biomechanical evaluations of the long-term stability dental implant using finite element modeling method: a systematic review. Adv. Prosthodont., 2022, vol. 14, no. 3, pp. 182-202, doi: 10.4047/jap.2022.14.3.182
  22. Iwata [et al.] Alteration of medullary dorsal horn neuronal activity following inferior alveolar nerve transectionin rats. J. Neurophysiol., 2001, vol. 86, no. 6, pp. 2868-2877, doi: 10.1152/jn.2001.86.6.2868
  23. Leelatian L. [et al.] Induction of osseointegration by nacre in pigs. Molecules, 2022, vol. 27, no. 9, 2653 p., doi: 10.3390/molecules27092653
  24. Mikic M. [et al.] The importance of correlation between CBCT analysis of bone density and primary stability when choosing the design of dental implants – Ex vivo study. Tomography, 2022, vol. 8, no. 3, pp. 1293-1306, doi: 3390/tomography8030107
  25. Misch C.E., Kircos L.T. Diagnostic imaging and techniques. Contemporary Implant. Dentistry., Mosby; St. Louis: 1999. pp. 73-87.

Correspondence to:  Е-mail: ip-c@yandex.ru