Stomatologist № 4 (31) – 2018, pp. 77-82                                                                                                                                 Scientific publication

Morphological and histochemical changes in periodontal tissues after odontopreparation of permanent teeth

S.V. Siraka, E.V. Shchetininа, G.D. Vafiadib, V.A. Grigoryanb
aMD, PhD, DMSci, Stavropol State Medical University, Stavropol, Russia
bbStavropol State Medical University, Stavropol, Russia

https://doi.org/10.32993/stomatologist.2018.4(31).14

ABSTRACT
The article presents the results of studying the morphofunctional and histochemical changes in tissue elements after the experimental preparation of sheep teeth.
The aim of the study is to investigate the morphofunctional and histochemical changes in the periodontal tissue elements after odontopreparation.
Materials and methods. The researchers have studied the dentoalveolar fragments of the lower jaw with central incisors taken from 16 sheep.
Results and discussion. The scientists established that damage of the periodontal tissues after odontopreparation leads to the development of an aseptic inflammatory process with a weak leukocyte and macrophage reaction and early activation of fibroblasts. By the 20th day of the experiment, the endothelium of the blood vessels and fibrin of collagen fibers showed the highest intensity of histochemical reactions, which was accompanied by an increase in the arithmetic mean of the periodontal vascular bed. The exudation was not accompanied by destructive processes, although it violated the morphology of collagen microfibrils. A high heterogeneity of fibroblasts was established with an increase in the indices of enzymatic activity in their cytoplasm during the entire period of the experiment.
Conclusions. The obtained data testify the high heterogeneity of fibroblasts capable to the rapid intracellular regulatory response by the amitotic division of the nuclei in the fibroblastic periodontal differon cells within 1 day after odontopreparation. An increase in the activity of enzymes in the cytoplasm of the periodontal fibroblasts of permanent teeth immediately after odontopreparation was noted, however, full recovery to the level of the control group values did not occur even by the 20th day.

Keywords: preparation of teeth, periodontium, inflammation, fibroblasts

References

  1. Grudyanov A.I., Zorin V.L., Pereverzev R.V., Zorina A.I., Bozo Ya.I. Jeffektivnost’ ispol’zovanija autofibroblastov pri hirurgicheskom lechenii parodontita [The effectiveness of the use of auto fibroblasts in the surgical treatment of periodontitis]. Geny i kletki. – Genes and cells, 2013, no. 3, pp. 72–77.
  2. Jumaev H.D., Shchetinina L.N. Kompleksnyj analiz populjacii tuchnyh kletok slizistoj obolochki desny v norme, pri melanoze i vospalitel’nyh zabolevanijah parodonta [Comprehensive analysis of the population of mast cells of the mucous membrane of the gums in normal conditions, with melanosis and inflammatory periodontal diseases]. Verhnevolzhskij medicinskij zhurnal. – Verkhnevolzhsky medical journal, 2017, no. 4, pp. 10–15.
  3. Merkulova N.A., Sedov V.M. Modificirovannye invivo sub#edinicy DNK-zavisimoj RNK-polimerazy III mlekopitajushhih [Modified in vivo subunits of mammalian DNA-dependent RNA polymerase III]. Citologija. – Cytology, 2015, no. 4 (57), pp. 254–259.
  4. Sleets A.A., Pereverzev R.V., Ibragimov I.M., Kodzokov B.A., Sirak S.V. Jeksperimental’noe opredelenie regeneratornogo potenciala kletok kostnogo mozga [Experimental determination of the regenerative potential of bone marrow cells]. Stomatologija dlja vseh. – Dentistry for all, 2012, no. 2, pp. 29–31.
  5. Gruber М., Gruber R., Agis H. Transforming growth factor-β1 increases sclerostin in fibroblasts of the periodontal ligament and the gingiva. Matters archive, 2017, no. 6 (20), pр. 213–228.
  6. Scutella F., Weinstein T., Zucchelli G., Testori T. A retrospective periodontal assessment of 137 teeth after featheredge preparation and gingittage. The International Journal of Periodontics, 2017, vol. 37, no. 6, pр. 791–800.
  7. Sirak S.V., Shchetinin E.V. Prevention of complications in patients suffering from pathological mandibular fractures due to bisphosphonate-associated osteonecroses. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2015, vol. 6, no. 5, pp. 1678–1684.
  8. Sirak S.W. Entschladen F., Shchetinin E.V., Grimm W.D. Low-level laser irradiation (810 nm) with toluidinblue photosensitizer promotes proliferation and differentiation of human oral fibroblasts evaluated in vitro. Journal of Clinical Periodontology, 2015, vol. 42, no. 17, pp. 328a–328.

Correspondence to: Е-mail: sergejsirak@yandex.ru