Dentist № 2 (53) – 2024, pp. 36-42                                                                                                                        SCIENTIFIC PUBLICATION

Cytotoxicity of some orthodontic adhesives

T.V. Gorlachevaa, O.S. Zalevskayab, T.N. Terekhovac

aMD, PhD, Associate Professor, Belarusian State Medical University, Minsk, Belarus
b Republican Scientific and Practical Center of Epidemiology and Microbiology, Minsk, Belarus
cMD, PhD, DMSci, Professor, Belarusian State Medical University, Minsk, Belarus

https://doi.org/10.32993/dentist.2024.2(53).3

ABSTRACT
It is known that composite materials that are used in dentistry, including orthodontic adhesives, can have a cytotoxic effect due to the content of methacrylates in them.
The aim of the study. Determine the cytotoxicity of orthodontic adhesives Heliosit orthodontics, Transbond Color Change Adhesive, Greengloo, Transbond Plus Self Etching Primer.
Objects and methods. The cytotoxicity of the studied materials was studied in unpolymerized, polymerized and polymerized forms, washed with distilled water, on a continuous culture of Vero-E6 cells using an inverted. Quantification of viability was performed by assessing the percentage of unstained (live) cells when counted on a Countess 3FL automatic cell counter. Descriptive statistics methods were used. The significance of differences in qualitative characteristics in groups was determined using the χ² criterion. The increase in the cytotoxicity of materials after their polymerization and washing with water was determined. Differences were considered statistically significant at p < 0.05.
Results and discussion. Average and moderate cytotoxicity of the studied materials was revealed. Polymerization of orthodontic adhesives and washing them with water leads to a decrease in the cytotoxicity of the studied samples: its increase ranges from -26.4 to -40.9 %.
Conclusion.  It is necessary to observe measures to prevent cytotoxic effects during work with orthodontic composites for both the patient and medical personnel.

Keywords: cytotoxicity of orthodontic adhesives

References

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Correspondence to:  Е-mail: terechova15t@gmail.com