Dentist № 3 (42) – 2021, pp. 16-19                                                                                                                SCIENTIFIC PUBLICATION

Express-test of the selected microflora in the contents of pathological periodontal pockets in patients with generalized periodontitis

L.N. Dedovaa, P.A. Semizhonb, О.V. Каndrukevichc, E.P. Scheslenokb

aMD, PhD, DMSci, Professor, Belarusian State Medical University, Minsk, Belarus
bPhD, The Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus
cMD, PhD, Associate Professor, Belarusian State Medical University, Minsk, Belarus

https://doi.org/10.32993/dentist.2021.3(42).8

ABSTRACT
Detailed diagnostic indicators for getting complete information of the dental status makes it possible to determine the disease in different periods of its development, effectively plan complex treatment and prevention.
The aim of the study. To determine the specific characteristics of clinical and laboratory studies of the microflora of T. denticola, P. gingivalis, B. forsythus in the contents of the pathological periodontal pocket in patients with periodontitis.
Objects and methods. Dental examination was performed on 60 patients with clinical signs of generalized periodontitis (main group) and 140 patients without periodontal disease (control group). We studied samples of biological material from the pathological periodontal pocket and / or the gingival sulcus using PCR and assessing the trypsin-like activity of the periopathogenic microflora.
Results and discussion. In samples from patients with periodontitis, DNA of periopathogenic bacteria was detected in diagnostically significant concentrations. As a result of a biochemical reaction in the area of application of samples from patients with periodontitis to the test strip, gray-blue spots were noted, indicating the presence of periopathogenic microorganisms, in contrast to the control group.
Conclusion. For effective determination of the pathogenic microflora of T. denticola, P. gingivalis, B. forsythus in the content of pathological dentogingival pocket in patients with periodontitis, it is advisable to use the molecular biological PCR method, as well as a biochemical express-test with the determination of the trypsinlike activity of the periopathogenic microflora.

Keywords: rapid diagnosis, periodontology, clinical and laboratory measures

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Correspondence to:  Е-mail: Dedova.bsmu@mail.ru